SamTrans uses a variety of buses to serve its customers throughout San Mateo County and into parts of San Francisco and Palo Alto. Articulated buses are assigned to routes that require a higher capacity. And, shorter buses are assigned to routes that move through tighter areas of the county.
| Total fixed-route revenue vehicles | 315 |
| Articulated coaches (60-foot) | 55 |
| Standard coaches (40-foot) | 186 |
| Battery Electric Buses (40-foot) | 17 |
| Fuel Cell Electric Buses (40-foot) | 1 |
| Standard coaches (35-foot) | 40 |
| Mini coaches (29-foot) | 16 |
| Paratransit vehicles | 70 |
| Cutaways | 46 |
| Transit Vans | 10 |
| Minivans | 14 |